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KMID : 0903619960370040607
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Journal of the Korean Society for Horticultural Science
1996 Volume.37 No. 4 p.607 ~ p.610
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Induction of Shoot Promordia of Hibiscus L. ´honghwarang´ by Suspension Cultures
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김기선/Kim, Ki Sun
유용권/유은하/Yoo, Yong Kweon/Yoo, Eun Ha
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Abstract
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This study was carried out to determine the optimum cultural condition and method for in vitro mass production of Hibiscus syriacus L. ´Honghwarang´. Callus was induced in MS medium supplemented with 2 ㎎/L 2,4-D in 8 weeks. Subsequent suspension culture for proliferation was conducted with MS liquid media supplemented with 2,4-D and TDZ, and callus growth and shoot primordia formation were examined after 4 weeks. Then suspension was transferred to hormone-free MS medium, and shoot primordia formation and shoot regeneration were examined after 4 weeks. During the first 4 weeks, treatment of 0.1 ㎎/L TDZ was the most effective on callus growth. Formation of shoot primordia was the best at the MS medium supplemented with 0.01 ㎎/L TDZ. When the suspensions cultured in each medium were transferred to hormone-free media, most shoot primordia were formed most from callus cultured at MS liquid media supplemented with TDZ 0.01 ㎎/L. Shoot primordia induced from culture at 0.1㎎/L TDZ were regenerated to shoot. We obtained about 30 calli from only one shoot-tip, and subsequently 20 to 30 shoot primordia were formed from each callus.
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